[Pathogen Identification for An Imported Case with African Trypanosomiasis].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
; 34(4): 350-4, 2016 Aug.
Article
en Zh
| MEDLINE
| ID: mdl-30148317
ABSTRACT
Objective:
To perform laboratory diagnosis for an imported case of human African trypanosomiasis and identify the pathogen.Methods:
Clinical and epidemiological information was collected. Blood and cerebrospinal fluid samples were collected, stained with Wright-Giemsa, and microscopically examined. Genomic DNA from the blood samples was amplified with primers specific for Trypanosoma sp. expression site-associated gene (ESAG), Trypanosoma brucei gambiense specific glycoprotein (TgsGP) and 18S rRNAï¼M18S-â ¡-Tbï¼ gene, and Trypanosoma brucei rhodesiense specific serum resistance associated ï¼SRAï¼ gene. Complete blood count, blood chemistry, and CSF examination were also conducted.Results:
The patient had a 4-month history of lower extremity weakness and swelling of surface lymph nodes. Physical examination showed somnolence, and occasional emotional abnormalities, accompanied by anemia (hemoglobin 85 g/L), electrolyte disturbance (sodium 124 mmol/L; chlorine 87 mmol/L) and significantly increased nonspecific immune globulin protein ï¼globulin 63 g/Lï¼. Epidemiological survey showed that the patient suffered insect bites and stings for several times during his work in the Republic of Gabon in Africa. Microscopic examination revealed flagella of trypanosome in peripheral blood. PCR amplification produced bands of 286, 308, and 150 bp with primers specific for ESAG, TgsGP and M18S-â ¡-Tb, respectively.Conclusion:
The patient was diagnosed with Trypanosoma brucei gambiense infection from the clinical information, epidemiological history, etiology and PCR results.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tripanosomiasis Africana
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
/
Humans
País/Región como asunto:
Africa
Idioma:
Zh
Revista:
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
Asunto de la revista:
PARASITOLOGIA
Año:
2016
Tipo del documento:
Article