Supercoil-dependent recognition of specific DNA sites by chromosomal protein HMG 2.
Biochem Biophys Res Commun
; 138(2): 910-6, 1986 Jul 31.
Article
en En
| MEDLINE
| ID: mdl-3017344
The ability of the chromosomal high mobility group protein HMG 2 to recognize supercoil-dependent structures within the chicken adult beta-globin gene was investigated by examining its ability to protect such sites from digestion by S1 nuclease. Low molar ratios of HMG 2 were found to be sufficient for complete inhibition of S1 cleavage of a supercoiled plasmid containing the globin gene. Furthermore, HMG 2 protected an S1 cleavage site within the 5'-flanking region of the globin gene, in preference to a palindromic S1 site within the plasmid vector.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
ADN Superhelicoidal
/
Globinas
/
Proteínas del Grupo de Alta Movilidad
/
Eritrocitos
/
Genes
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1986
Tipo del documento:
Article
Pais de publicación:
Estados Unidos