New CeF3-ZnO nanocomposites for self-lighted photodynamic therapy that block adenocarcinoma cell life cycle.
Nanomedicine (Lond)
; 13(18): 2311-2326, 2018 09.
Article
en En
| MEDLINE
| ID: mdl-30198424
ABSTRACT
AIM:
To synthesize and characterize the performances of a new all-inorganic nanocomposite (NC) for self-lighted photodynamic therapy against cancer. This NC could allow radiotherapy doses to be reduced, as it enhances the effects of x-rays, generating cytotoxic reactive oxygen species as singlet oxygen. MATERIALS &METHODS:
The proposed NC combines CeF3 and ZnO; CeF3 absorbs 6-MeV x-rays and activates the photosensitizer ZnO. Characterization is performed by transmission electron microscopy (TEM), scanning-TEM, energy dispersive x-ray spectrometry and fluorescence spectroscopies. Efficiency on human adenocarcinoma cells (A549) was tested by fluorescence spectroscopy, cytofluorimetry, viability assays, clonogenic assays, cell cycle progression assays.RESULTS:
NC blocks A549's cell cycle before mitosis in the dark. Upon low-dose x-ray irradiation (2 Gy), reactive oxygen species/singlet oxygen are generated, further blocking cell cycle and reducing viability by 18% with respect to the sum of x-ray irradiation and NC dark activity.CONCLUSION:
These novel NCs promise to reduce doses in radiotherapy, helping to reduce unwanted side effects.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fotoquimioterapia
/
Óxido de Zinc
/
Adenocarcinoma
/
Cerio
/
Nanocompuestos
/
Fluoruros
Límite:
Humans
Idioma:
En
Revista:
Nanomedicine (Lond)
Año:
2018
Tipo del documento:
Article
País de afiliación:
Italia