Protein fishing from single live cells.
J Nanobiotechnology
; 16(1): 67, 2018 Sep 11.
Article
en En
| MEDLINE
| ID: mdl-30205820
ABSTRACT
Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to 'fish' native cytoplasmic or nuclear proteins from single mammalian cells, without compromising cell viability, followed by ex cellulo quantitative detection. Our work paves the way for spatiotemporally-controlled, quantitative, live, single-cell proteomics.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas
/
Proteómica
/
Análisis de la Célula Individual
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Nanobiotechnology
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos