An optoelectronic tongue based on an array of gold and silver nanoparticles for analysis of natural, synthetic and biological antioxidants.

ABSTRACT

A colorimetric array, which can discriminate 20 food antioxidants of natural, synthetic and biological groups, is described. It consists of gold and silver nanoparticles that were synthesized using six different reducing and/or capping agents. The function of the array relies on the interaction of the antioxidants with the nanoparticles which causes aggregation or morphological changes. This, in turn, causes a change in the sensors' colors. The array produces a unique combination of colors for each antioxidant. The resulting colorations are determined by recording the absorbances of the arrays at wavelengths of 405, 450, 490 and 630 nm, or by capturing the images with a digital camera. The discriminatory ability of the array is investigated by principle component analysis and hierarchical cluster analysis. The method was applied to quantitative assay of gallic acid, caffeic acid, catechin, dopamine, citric acid, butylated hydroxytoluene and ascorbic acid. The respective limits of detection are 4.2, 13, 53, 6.9, 47, 3.5 and 43 nM, respectively. The simultaneous determination of 5 different antioxidants is achieved utilizing partial least square regression. The root mean square errors for prediction of the test set are 0.0650, 0.0782, 0.811, 0.0206 and 0.135 nM for gallic acid, catechin, butylated hydroxytoluene, dopamine, and ascorbic acid, respectively. This method demonstrates excellent potential for analysis of antioxidants in beverages such as tea and lemon juice. Graphical abstract Schematic of a method for the simultaneous determination of several antioxidants based on changes in the color of gold and silver nanoparticles. The antioxidants cause aggregation and/or morphological changes which can be detected by using both image analysis or by colorimetry.