Live Imaging of mRNA Transcription in Drosophila Embryos.
Methods Mol Biol
; 1863: 165-182, 2018.
Article
en En
| MEDLINE
| ID: mdl-30324598
ABSTRACT
Live imaging has been used in recent years for the understanding of dynamic processes in biology, such as embryo development. This was made possible by a combination of advancements in microscopy, leading to improved signal-to-noise ratios and better spatial and temporal resolutions, and by the development of new fluorescence markers, allowing for the quantification of protein expression and transcriptional dynamics in vivo. Here we describe a general protocol, which can be used in standard confocal microscopes to image early Drosophila melanogaster embryos, in order to learn about the transcriptional dynamics of a fluorescently labeled RNA.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transcripción Genética
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Procesamiento de Imagen Asistido por Computador
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ARN Mensajero
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Microscopía Confocal
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Drosophila melanogaster
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Embrión no Mamífero
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2018
Tipo del documento:
Article
País de afiliación:
Francia