Characterization of growth hormone disulfide-linked molecular isoforms during post-exercise release vs nocturnal pulsatile release reveals similar milieu composition.
Growth Horm IGF Res
; 42-43: 102-107, 2018.
Article
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| MEDLINE
| ID: mdl-30399477
OBJECTIVE: To characterize the influence of mode (aerobic/resistance) and volume of exercise (moderate/high) on circulating GH immediately post-exercise as well as following the onset of sleep. DESIGN: This study used repeated measures in which subjects randomly completed 5 separate conditions: control (no exercise), moderate volume resistance exercise (MR), high-volume resistance exercise (HR), moderate volume aerobic exercise (MA), and high volume aerobic exercise (HA). METHODS: Subjects had two overnight stays on each of the 5 iterations. Serial blood draws began as soon as possible after the completion of the exercise session. Blood was obtained every 20â¯min for 24-h. GH was measured using a chemiluminescent immunoassay. Pooled samples representing post exercise (PE) and first nocturnal pulse (NP) were divided into two aliquots. One of these aliquots was chemically reduced by adding 10â¯mM glutathione (GSH) to break down disulfide-linked aggregates. RESULTS: No differences were observed when pooling GH response at post-exercise (2.02⯱â¯0.21) and nocturnal pulse (2.63⯱â¯0.51; pâ¯=â¯.32). Pairwise comparisons revealed main effect differences between controls (1.19⯱â¯0.29) and both MA (2.86⯱â¯0.31; pâ¯=â¯.009) and HA (3.73⯱â¯0.71; pâ¯=â¯.001). Both MA (pâ¯=â¯.049) and HA (pâ¯=â¯.035) responses were significantly larger than the MR stimulus (1.96⯱â¯0.28). With GSH reduction, controls significantly differed from MA (pâ¯=â¯.018) and HA (pâ¯=â¯.003) during PE, but only differed from HA (pâ¯=â¯.003) during NP. CONCLUSIONS: This study demonstrated similar GH responses to exercise and nocturnal pulse, indicating that mode and intensity of exercise does not proportionately affect GH dimeric isoform concentration.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sueño
/
Ejercicio Físico
/
Hormona de Crecimiento Humana
/
Disulfuros
/
Fuerza Muscular
/
Entrenamiento de Fuerza
Tipo de estudio:
Clinical_trials
Límite:
Humans
Idioma:
En
Revista:
Growth Horm IGF Res
Asunto de la revista:
ENDOCRINOLOGIA
Año:
2018
Tipo del documento:
Article
Pais de publicación:
Reino Unido