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Impacts of soil salinity on Bt protein concentration in square of transgenic Bt cotton.
Wang, Yong-Hui; Gao, Jin; Sun, Ming-Fa; Chen, Jian-Ping; Zhang, Xiang; Chen, Yuan; Chen, De-Hua.
Afiliación
  • Wang YH; Institute of Agricultural Sciences of Jiangsu Coastal Area, Observation and Experimental Station of Saline Land of Costal Area, Ministry of Agriculture, Yancheng, P. R. China.
  • Gao J; Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou, P. R. China.
  • Sun MF; Institute of Agricultural Sciences of Jiangsu Coastal Area, Observation and Experimental Station of Saline Land of Costal Area, Ministry of Agriculture, Yancheng, P. R. China.
  • Chen JP; Institute of Agricultural Sciences of Jiangsu Coastal Area, Observation and Experimental Station of Saline Land of Costal Area, Ministry of Agriculture, Yancheng, P. R. China.
  • Zhang X; Institute of Agricultural Sciences of Jiangsu Coastal Area, Observation and Experimental Station of Saline Land of Costal Area, Ministry of Agriculture, Yancheng, P. R. China.
  • Chen Y; Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou, P. R. China.
  • Chen DH; Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou, P. R. China.
PLoS One ; 13(11): e0207013, 2018.
Article en En | MEDLINE | ID: mdl-30403755
ABSTRACT
Insect-resistance of transgenic Bacillus thuringiensis (Bt) cotton varies among plants organs and with different environmental conditions. The objective of this study was to examine the influence of soil salinity on Bt protein concentration in cotton squares and to elucidate the potential mechanism of Bt efficacy reduction. Two cotton cultivars (NuCOTN 33B and CCRI 07, salt-sensitive and salt-tolerant) were subjected to salinity stress under four natural saline levels in field conditions in 2015 and 2016 and seven regimes of soil salinity ranged from 0.5 to 18.8 dS m-1 in greenhouse conditions in 2017. Results of field studies revealed that Bt protein content was not significantly changed at 7.13 dS m-1 salinity, but exhibited a significant drop at the 10.41 and 14.16 dS m-1 salinity. The greenhouse experiments further showed similar trends that significant declines of the insecticidal protein contents in squares were detected when soil salinity exceeded 9.1 dS m-1. Meanwhile, high salinity resulted in significant reduction in contents of soluble protein and total nitrogen, activities of nitrate reductase (NR), glutamine synthetase (GS) and glutamic-pyruvic transaminase (GPT), but increased amino acid content, activities of protease and peptidase in cotton squares. High salinity also decreased root vigor (RV), root total absorption area (RTA) and root active absorption area (RAA). The extent of decrease of Bt protein content was more pronounced in NuCOTN 33B than CCRI 07, and CCRI07 exhibited stronger enzymes activities involved in square protein synthesis and higher levels of RV, RTA and RAA. Therefore, the results of our present study indicated that insecticidal protein expression in cotton squares were significantly affected by higher salinity (equal to or higher than 9.1 dS m-1), reduced protein synthesis and increased protein degradation in squares and reduced metabolic activities in roots might lead to the decrease of Bt protein content in squares.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Suelo / Proteínas Bacterianas / Gossypium / Endotoxinas / Proteínas Hemolisinas Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article Pais de publicación: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Suelo / Proteínas Bacterianas / Gossypium / Endotoxinas / Proteínas Hemolisinas Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article Pais de publicación: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA