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Long-term effectiveness of local BM-MSCs for skeletal muscle regeneration: a proof of concept obtained on a pig model of severe radiation burn.
Linard, Christine; Brachet, Michel; L'homme, Bruno; Strup-Perrot, Carine; Busson, Elodie; Bonneau, Michel; Lataillade, Jean-Jacques; Bey, Eric; Benderitter, Marc.
Afiliación
  • Linard C; Institute of Radiological Protection and Nuclear Safety, B.P. n°17, F-92262, Fontenay-aux-Roses, France. christine.linard@irsn.fr.
  • Brachet M; Unité de Thérapie Tissulaire et Traumatologie de Guerre, Institut de Recherche Biomédicale des Armées, Clamart, France. christine.linard@irsn.fr.
  • L'homme B; Department of Plastic Surgery, Military Hospital of Percy, Clamart, France.
  • Strup-Perrot C; Institute of Radiological Protection and Nuclear Safety, B.P. n°17, F-92262, Fontenay-aux-Roses, France.
  • Busson E; Institute of Radiological Protection and Nuclear Safety, B.P. n°17, F-92262, Fontenay-aux-Roses, France.
  • Bonneau M; Unité des Médicaments de Thérapie Innovante, Centre de Transfusion Sanguine des Armées, Clamart, France.
  • Lataillade JJ; Centre of Research in Interventional Imaging, National Institut of Agronomic Research, Jouy-en-Josas, France.
  • Bey E; Unité de Thérapie Tissulaire et Traumatologie de Guerre, Institut de Recherche Biomédicale des Armées, Clamart, France.
  • Benderitter M; Department of Plastic Surgery, Military Hospital of Percy, Clamart, France.
Stem Cell Res Ther ; 9(1): 299, 2018 11 08.
Article en En | MEDLINE | ID: mdl-30409227
BACKGROUND: Medical management of the severe musculocutaneous radiation syndrome involves surgical intervention with debridement of necrotic tissue. Even when skin excision is replaced by specific plastic surgery, treatment of the muscle radiation injury nonetheless remains difficult, for it involves a massive muscle defect in an unpredictable environment, subject to inflammatory waves weeks to months after irradiation, which delay healing and predispose the patient to the development of fibrous scar tissue. In this study, we investigated the long-term effect of local injections of bone marrow-derived mesenchymal stromal cells (BM-MSCs), combined with plastic surgery, to treat muscle necrosis in a large animal model. METHODS: Three months after irradiation to the rump, minipigs were treated by excision of necrotic muscle tissue, vascularized flap surgery, and four injections with or without local autologous BM-MSCs, performed weekly. The quality of the muscle wound healing was examined 1 year post-surgery. RESULTS: The skeletal muscle surgery without MSC treatment led to permanent deposition of collagen 1 and 3, decreased myofiber diameter, failed muscle fiber regeneration, a reduced number of capillaries, and the accumulation of high calcium and fat. In animals treated by surgery and MSC injections, these indicators were substantially better and demonstrated established regeneration. MSC therapy acts at several levels by stimulating growth factors such as VEGF, which is involved in angiogenesis and satellite cell pool maintenance, and creating a macrophage M1/M2 balance. CONCLUSION: Thus, cell therapy using BM-MSCs is an effective and safe way to improve recovery of irradiation-induced skeletal muscle damage without signs of long-term degeneration.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos por Radiación / Regeneración / Quemaduras / Células de la Médula Ósea / Músculo Esquelético / Trasplante de Células Madre Mesenquimatosas / Células Madre Mesenquimatosas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Stem Cell Res Ther Año: 2018 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos por Radiación / Regeneración / Quemaduras / Células de la Médula Ósea / Músculo Esquelético / Trasplante de Células Madre Mesenquimatosas / Células Madre Mesenquimatosas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Stem Cell Res Ther Año: 2018 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido