Autophagy induced by ionizing radiation promotes cell death over survival in human colorectal cancer cells.
Exp Cell Res
; 374(1): 29-37, 2019 01 01.
Article
en En
| MEDLINE
| ID: mdl-30412717
ABSTRACT
Autophagy is commonly described as a cell survival mechanism and has been implicated in chemo- and radioresistance of cancer cells. Whether ionizing radiation induced autophagy triggers tumor cell survival or cell death still remains unclear. In this study the autophagy related proteins Beclin1 and ATG7 were tested as potential targets to sensitize colorectal carcinoma cells to ionizing radiation under normoxic, hypoxic and starvation conditions. Colony formation, apoptosis and cell cycle analysis revealed that knockdown of Beclin1 or ATG7 does not enhance radiosensitivity in HCT-116 cells. Furthermore, ATG7 knockdown led to an increased survival fraction under oxygen and glutamine starvation, indicating that ionizing radiation indeed induces autophagy which, however, leads to cell death finally. These results highlight that inhibition of autophagic pathways does not generally increase therapy success but may also lead to an unfavorable outcome especially under amino acid and oxygen restriction.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Radiación Ionizante
/
Autofagia
/
Neoplasias Colorrectales
/
Apoptosis
Límite:
Humans
Idioma:
En
Revista:
Exp Cell Res
Año:
2019
Tipo del documento:
Article
País de afiliación:
Alemania