UPLC/QTOFMS based plasma metabolomics and clinical characteristics of polycystic ovarian syndrome.
Mol Med Rep
; 19(1): 280-292, 2019 01.
Article
en En
| MEDLINE
| ID: mdl-30431132
ABSTRACT
The present study aimed to develop novel diagnostic methods for polycystic ovarian syndrome (PCOS) by screening and identifying specific PCOSassociated metabolic markers using plasma metabolomics. Ultraperformance liquid chromatography/quadrapoletime of flightmass spectrometry was adopted to establish the plasma metabolic fingerprint of 49 patients and 50 normal controls, in order to screen the potential metabolic markers. In addition, these markers were integrated with the clinical indexes, followed by focused analysis to obtain diagnostic markers. The present results demonstrated that not only was the concentration of palmitoyl sphingomyelin in plasma of patients with PCOS significantly increased; however, a statistically significant difference between the two PCOS subgroups was additionally demonstrated. At the same time, the concentrations of cyclic guanosine monophosphate (cGMP) and dehydroepiandrosterone sulphate in the plasma of patients of the subgroup 1 were significantly elevated. These markers were additionally integrated with the clinical index number of follicles in the left ovary and highdensity lipoprotein (HDLC), followed by receiver operating characteristic curve analysis, which demonstrated a diagnostic accuracy of ~90% in the control and the two subgroups. The integrated marker system consisting of palmitoyl sphingomyelin, cGMP and androsterone sulfate, as well as the number of left follicles and HDLC may be used for the accurate diagnosis and classification of PCOS. These results confirmed that the abnormalities in hormone metabolism and lipid metabolism disorder were primarily involved in the onset of PCOS.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plasma
/
Síndrome del Ovario Poliquístico
/
Biomarcadores
/
Cromatografía Liquida
/
Espectrometría de Masa por Ionización de Electrospray
/
Metabolómica
Tipo de estudio:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Límite:
Adolescent
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Adult
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Female
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Humans
Idioma:
En
Revista:
Mol Med Rep
Año:
2019
Tipo del documento:
Article