Carvacrol ameliorates experimental autoimmune encephalomyelitis through modulating pro- and anti-inflammatory cytokines.
Life Sci
; 219: 257-263, 2019 Feb 15.
Article
en En
| MEDLINE
| ID: mdl-30472298
ABSTRACT
AIM:
The inflammatory process is a key step in multiple sclerosis (MS) development. Carvacrol exhibits various anti-inflammatory properties. We aimed to assess the Carvacrol effects on clinical manifestations and production of pro-inflammatory (IFN-γ, IL-6 and IL-17) and anti-inflammatory (TGF-ß, IL-4, and IL-10) cytokines in experimental autoimmune encephalomyelitis (EAE) as MS animal model. MAINMETHODS:
EAE mice were treated with 5, 10â¯mg/kg dose of Carvacrol or vehicle, as the control EAE group, every other day until day-21 post EAE induction. On day22, the leukocyte infiltration within the CNS was estimated using hematoxylin-eosin staining. The cytokine production by splenocytes was determined after in vitro stimulating with myelin oligodendrocyte protein (MOG). KEYFINDINGS:
The EAE clinical scores in 5 and 10â¯mg/kg Carvacrol-treated mice were lower than untreated group (Pâ¯<â¯0.001 and Pâ¯<â¯0.01, respectively). The amounts of IFN-γ and IL-6 production by splenocytes of 5 and 10â¯mg/kg Carvacrol-administered mice were lower than control group (Pâ¯<â¯0.001, and Pâ¯<â¯0.01 for IFN-γ respectively; Pâ¯Ëâ¯0.05 for IL-6). Splenocytes of 5 and 10â¯mg/kg Carvacrol-treated mice produced higher levels of TGF-ß than untreated mice (Pâ¯<â¯0.001). in splenocytes of 5â¯mg/kg Carvacrol-treated group the IL-10 production was higher while IL-17 secretion was lower than control group (both with Pâ¯<â¯0.01).SIGNIFICANCE:
Carvacrol exhibits modulatory effects on expression of pro- and anti-inflammatory cytokines. It ameliorates EAE clinical and pathological consequences and therefore its potentials may be considered in treating MS patients.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Citocinas
/
Monoterpenos
/
Encefalomielitis Autoinmune Experimental
/
Antiinflamatorios
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Life Sci
Año:
2019
Tipo del documento:
Article
País de afiliación:
Irán