Gold nanorods/siRNA complex administration for knockdown of PARP-1: a potential treatment for perinatal asphyxia.
Int J Nanomedicine
; 13: 6839-6854, 2018.
Article
en En
| MEDLINE
| ID: mdl-30498346
ABSTRACT
BACKGROUND:
Perinatal asphyxia interferes with neonatal development, resulting in long-term deficits associated with systemic and neurological diseases. Despite the important role of poly (ADP-ribose) polymerase 1 (PARP-1) in the regulation of gene expression and DNA repair, overactivation of PARP-1 in asphyxia-exposed animals worsens the ATP-dependent energetic crisis. Inhibition of PARP-1 offers a therapeutic strategy for diminishing the effects of perinatal asphyxia.METHODS:
We designed a nanosystem that incorporates a specific siRNA for PARP-1 knockdown. The siRNA was complexed with gold nanorods (AuNR) conjugated to the peptide CLPFFD for brain targeting.RESULTS:
The siRNA was efficiently delivered into PC12 cells, resulting in gene silencing. The complex was administered intraperitoneally in vivo to asphyxia-exposed rat pups, and the ability of the AuNR-CLPFFD/siRNA complex to reach the brain was demonstrated.CONCLUSION:
The combination of a nanosystem for delivery and a specific siRNA for gene silencing resulted in effective inhibition of PARP-1 in vivo.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Asfixia
/
Poli(ADP-Ribosa) Polimerasas
/
ARN Interferente Pequeño
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Nanotubos
/
Técnicas de Silenciamiento del Gen
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Oro
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Int J Nanomedicine
Año:
2018
Tipo del documento:
Article