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In vitro synthesis of the human calcium transporter Letm1 within cell-sized liposomes and investigation of its lipid dependency.
Okamura, Kosuke; Matsushita, Shuhei; Kato, Yasuhiko; Watanabe, Hajime; Matsui, Aiko; Oka, Toshihiko; Matsuura, Tomoaki.
Afiliación
  • Okamura K; Department of Biotechnology, Division of Advance Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
  • Matsushita S; Department of Biotechnology, Division of Advance Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
  • Kato Y; Department of Biotechnology, Division of Advance Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
  • Watanabe H; Department of Biotechnology, Division of Advance Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
  • Matsui A; Department of Life Science, Rikkyo University, 3-34-1 Nishi-Ikebukuro, Toshima-Ku, Tokyo 171-8501, Japan.
  • Oka T; Department of Life Science, Rikkyo University, 3-34-1 Nishi-Ikebukuro, Toshima-Ku, Tokyo 171-8501, Japan.
  • Matsuura T; Department of Biotechnology, Division of Advance Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: matsuura_tomoaki@bio.eng.osaka-u.ac.jp.
J Biosci Bioeng ; 127(5): 544-548, 2019 May.
Article en En | MEDLINE | ID: mdl-30503650
ABSTRACT
The human mitochondrion-derived calcium transporter Letm1 was synthesized by reconstituted in vitro transcription-translation (IVTT) in cell-sized liposomes and the dependency of Letm1 on phospholipid composition was investigated. Components for IVTT were encapsulated into cell-sized vesicles together with the DNA encoding Letm1, thereby preparing proteoliposomes. The synthesis of Letm1 and pH-dependent calcium transport activity were confirmed by flow cytometry. Finally, we investigated the effect of phospholipid composition on Letm1 transport activity and found that cardiolipin present in the mitochondrial membrane plays an important role on the transport activity of Letm1.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfolípidos / Biosíntesis de Proteínas / Proteínas de Unión al Calcio / Proteínas de la Membrana Límite: Humans Idioma: En Revista: J Biosci Bioeng Asunto de la revista: ENGENHARIA BIOMEDICA / MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Japón
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfolípidos / Biosíntesis de Proteínas / Proteínas de Unión al Calcio / Proteínas de la Membrana Límite: Humans Idioma: En Revista: J Biosci Bioeng Asunto de la revista: ENGENHARIA BIOMEDICA / MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Japón