A subset of CD1c+ dendritic cells is increased in patients with tuberculosis and promotes Th17â¯cell polarization.
Tuberculosis (Edinb)
; 113: 189-199, 2018 12.
Article
en En
| MEDLINE
| ID: mdl-30514502
ABSTRACT
The role of primary subsets of DCs in Mycobacterium tuberculosis infection in humans is incompletely understood. In this study, we identified a CD1c DC subset with phenotype of CD1c+CD11c+CD19-CD11b+ that was significantly increased in tuberculous pleural effusions and in peripheral blood from patients with TB compared with that from healthy controls (pâ¯<â¯0.0001). Sputum smear/culture-positive patients with tuberculosis had significantly higher frequency of CD1c+CD11b+ DC subset than sputum smear/culture-negative patients (pâ¯<â¯0.0001). After effective anti-TB chemotherapy, the frequency of CD1c+CD11b+ DC subset in peripheral blood and tuberculous pleural effusions was decreased. CD1c+CD11b+ DC subset from tuberculous pleural effusions expressed higher levels of TLR2, TLR4, CD172a, CD206 and FcεRâ
, but lower levels of CD80, CD83 and CD86 compared with CD1c+CD11b- DC subset. Expression of IL-1ß, IL-6, IL-8, IL-23, TNF-α, IFN-γ and TGF-ß mRNA in CD1c+CD11b+ DCs was higher than in CD1c+CD11b- DC subset. Co-culture of autologous naive CD4+ T cells with sorted CD1c+CD11b+ DCs expressed significantly increased levels of IL-17A and RORγt transcripts as compared with those co-cultured with CD11b- subset. In conclusion, a CD1c+CD11b+ DC subset with elevated frequency in patients with tuberculosis was identified and it promoted Th17â¯cell differentiation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tuberculosis Pulmonar
/
Células Dendríticas
/
Glicoproteínas
/
Antígenos CD1
/
Células Th17
/
Inmunidad Innata
/
Pulmón
/
Mycobacterium tuberculosis
Tipo de estudio:
Observational_studies
Límite:
Humans
Idioma:
En
Revista:
Tuberculosis (Edinb)
Año:
2018
Tipo del documento:
Article
País de afiliación:
China