Inhibitory effects of polysaccharide from Diaphragma juglandis fructus on α-amylase and α-d-glucosidase activity, streptozotocin-induced hyperglycemia model, advanced glycation end-products formation, and H2O2-induced oxidative damage.

ABSTRACT

In present study, the in vitro and in vivo hemolysis inhibitory, protective effect against reactive oxygen species (ROS) induced oxidative damage in L02 cells, hypoglycemic, and antiglycation activities of DJP-2, a pure polysaccharide fraction from Diaphragma juglandis fructus, were investigated. Results demonstrated that DJP-2 showed remarkable hemolysis inhibitory activity. Pretreatment with DJP-2 markedly weakened the oxidative damage induced by H2O2 in hepatic L02 cells via strengthening the cell viability. DJP-2 also showed clear in vivo and in vitro hypoglycemic activities. Besides, DJP-2 with the concentration of 3 mg/mL exerted more significant antiglycation activities than aminoguanidine during 30 days of incubation. The results obtained in this study would be beneficial for the application of DJP-2 to treat various diseases related to oxidative stress and AGEs. The elucidation of the potential bioactivities of DJP-2 will facilitate its further study and application in the functional food industry and pharmaceuticals industry.