Cholinergic M4 receptors are involved in morphine-induced expression of behavioral sensitization by regulating dopamine function in the nucleus accumbens of rats.
Behav Brain Res
; 360: 128-133, 2019 03 15.
Article
en En
| MEDLINE
| ID: mdl-30529589
ABSTRACT
Repeated administration of morphine profoundly influences the dopaminergic and cholinergic systems in the nucleus accumbens [including the shell of the nucleus accumbens (NAcS)]. Further, dopamine release is regulated by the cholinergic system, especially the M4 receptor. Drug priming is one of the main factors that induces relapse in drug addiction. The present study first investigated how activation of the M4 receptor in the NAcS affects the expression of morphine-induced behavioral sensitization, through the administration of an M4 agonist (LY2033298) and antagonist (tropicamide), as well as a combination of an acetylcholinesterase inhibitor and M4 antagonist (huperzine-A + tropicamide). Additionally, the influence of a dopamine receptor agonist, in conjunction with an M4 agonist (i.e., SKF38393 + LY2033298), was also examined. Behavioral sensitization was established by exposure to 5 mg/kg morphine once every three days for a total of three exposures. The expression of behavioral sensitization was challenged by 5 mg/kg morphine. Results showed that (1) microinjection of the M4 receptor agonist LY2033298 (0.2 µg/side), but not the antagonist tropicamide (5, 10, or 20 µM/side) into the NAcS blocked the expression of behavioral sensitization; (2) tropicamide (20 µM/side) reversed the inhibition effect of huperzine-A on this behavior; and (3) SKF38393 (1 µg/side) reversed the inhibitory effect of LY2033298 on the expression of morphine-induced behavioral sensitization. These results suggest that the cholinergic M4 receptor in the NAcS plays an important role in the morphine-induced expression of behavioral sensitization through the regulation of dopamine function in rats.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Síntomas Conductuales
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Dopamina
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Receptor Muscarínico M4
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Morfina
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Narcóticos
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Núcleo Accumbens
Límite:
Animals
Idioma:
En
Revista:
Behav Brain Res
Año:
2019
Tipo del documento:
Article