Role of microparticles derived from monocytes, endothelial cells and platelets in the exacerbation of COPD.
Int J Chron Obstruct Pulmon Dis
; 13: 3749-3757, 2018.
Article
en En
| MEDLINE
| ID: mdl-30532530
ABSTRACT
BACKGROUND:
Microparticles (MPs) are shedding membrane vesicles released from activated blood and endothelial cells under inflammatory conditions. The role of endothelial MPs (EMPs) in pathophysiology of COPD is relatively well known. However, the release and function of MPs of other cellular origins, eg, platelets, red blood cells and leukocytes, are not clearly evaluated in COPD.PURPOSE:
The aim of this study was to measure EMPs and other cell-derived circulating MPs in stable and exacerbated COPD patients. PATIENTS ANDMETHODS:
A total of 50 patients with COPD and 19 healthy volunteers were enrolled in the study. EMPs (CD31+, CD62E+) and platelet-derived (CD61+, CD41+, CD42a+, PAC1+), red blood cell-derived (GlyA+) and leukocyte-derived (CD45+, CD13+, CD14+, CD56+) MPs were measured. Flow cytometry (FC) was performed on Beckman Coulter FC500 analyzer. MP reference gate was set using 0.3-0.5-0.9 µm microbeads with MP size gates of 0.5-1.0 µm.RESULTS:
All the measured MPs were significantly (P<0.001) higher in COPD patients than in the controls. Furthermore, CD62E+, CD41+, CD42a+ and CD14+ MP values were significantly (P<0.001) increased in exacerbated COPD compared to stable COPD. These MPs showed significant (P<0.001) inverse correlation with FEV1/FVC, as well.CONCLUSION:
In this study, we describe a reliable flow cytometric assay for MP analysis that was successfully applied in COPD. Besides EMPs, COPD is accompanied by an increased concentration of various MPs in the systemic circulation; particularly, platelet- and monocyte-derived MPs seem to be important in exacerbation.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plaquetas
/
Monocitos
/
Enfermedad Pulmonar Obstructiva Crónica
/
Células Endoteliales
/
Micropartículas Derivadas de Células
Tipo de estudio:
Observational_studies
Límite:
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Int J Chron Obstruct Pulmon Dis
Año:
2018
Tipo del documento:
Article
País de afiliación:
Hungria