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Histamine H3 receptor antagonist Clobenpropit protects propofol-induced apoptosis of hippocampal neurons through PI3K/AKT pathway.
He, W; Yuan, Q-H; Zhou, Q.
Afiliación
  • He W; Department of Anaesthesiology, Mianyang Central Hospital, Mianyang, China. 278523276@qq.com.
Eur Rev Med Pharmacol Sci ; 22(22): 8013-8020, 2018 11.
Article en En | MEDLINE | ID: mdl-30536350
ABSTRACT

OBJECTIVE:

The aim of the study was to explore whether histamine H3 receptor antagonist Clobenpropit could protect propofol-induced neurotoxicity in hippocampal neurons. MATERIALS AND

METHODS:

Hippocampal neurons were extracted from neonatal rats and induced with propofol. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay was performed to detect apoptotic rate of neurons. Western blot was conducted to detect protein levels of cleaved-caspase-3 and Bax/Bcl2. After LY294002 treatment, the PI3K pathway antagonist was applied in neurons, protein levels of cleaved-caspase-3 and Bax/Bcl2 were detected by Western blot as well.

RESULTS:

Propofol treatment remarkably induced neuronal apoptosis. Clobenpropit alleviated cell apoptosis induced by propofol. Protein expressions of cleaved-caspase-3 and Bax/Bcl2 were remarkably downregulated in neurons treated with Clobenpropit. LY294002 induction remarkably reverses the protective role of Clobenpropit in neuronal apoptosis, manifesting as downregulated PI3K and p-AKT after LY294002 treatment.

CONCLUSIONS:

Clobenpropit protects propofol-induced neuronal apoptosis through activating PI3K/AKT pathway.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tiourea / Propofol / Fosfatidilinositol 3-Quinasas / Proteínas Proto-Oncogénicas c-akt / Antagonistas de los Receptores Histamínicos H3 / Hipocampo / Imidazoles Límite: Animals Idioma: En Revista: Eur Rev Med Pharmacol Sci Asunto de la revista: FARMACOLOGIA / TOXICOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tiourea / Propofol / Fosfatidilinositol 3-Quinasas / Proteínas Proto-Oncogénicas c-akt / Antagonistas de los Receptores Histamínicos H3 / Hipocampo / Imidazoles Límite: Animals Idioma: En Revista: Eur Rev Med Pharmacol Sci Asunto de la revista: FARMACOLOGIA / TOXICOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: China