International Space Station conditions alter genomics, proteomics, and metabolomics in Aspergillus nidulans.
Appl Microbiol Biotechnol
; 103(3): 1363-1377, 2019 Feb.
Article
en En
| MEDLINE
| ID: mdl-30539259
ABSTRACT
The first global genomic, proteomic, and secondary metabolomic characterization of the filamentous fungus Aspergillus nidulans following growth onboard the International Space Station (ISS) is reported. The investigation included the A. nidulans wild-type and three mutant strains, two of which were genetically engineered to enhance secondary metabolite production. Whole genome sequencing revealed that ISS conditions altered the A. nidulans genome in specific regions. In strain CW12001, which features overexpression of the secondary metabolite global regulator laeA, ISS conditions induced the loss of the laeA stop codon. Differential expression of proteins involved in stress response, carbohydrate metabolic processes, and secondary metabolite biosynthesis was also observed. ISS conditions significantly decreased prenyl xanthone production in the wild-type strain and increased asperthecin production in LO1362 and CW12001, which are deficient in a major DNA repair mechanism. These data provide valuable insights into the adaptation mechanism of A. nidulans to spacecraft environments.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Estrés Fisiológico
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Regulación Fúngica de la Expresión Génica
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Metabolismo de los Hidratos de Carbono
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Metabolismo Secundario
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Genes Fúngicos
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Estados Unidos