Preparation of restricted-access material precolumns by grafting δ-gluconolactone onto a hybrid silica monolithic column for on-line solid-phase extraction of tetracycline residues from milk.
J Sep Sci
; 42(4): 843-850, 2019 Feb.
Article
en En
| MEDLINE
| ID: mdl-30569534
ABSTRACT
A restricted-access material-hybrid monolithic column was prepared based on single-component organosiloxane and dynamic grafting of δ-gluconolactone for on-line solid phase extraction of tetracycline antibiotic residues from milk. The hybrid monolithic column was prepared in a stainless-steel chromatographic column using methyltrimethoxysilane as the single precursor. δ-Gluconolactone was covalently coupled to aminopropyl derivatized hybrid monolithic column, which formed hydrophilic structures on the surface of the pore of the restricted-access material-hybrid monolithic column. The columns were characterized by scanning electron microscopy, thermogravimetric analysis, Fourier transform infrared spectroscopy, nitrogen adsorption, contact angle analysis, dynamic adsorption, and chromatographic performance evaluation. The restricted-access material-hybrid monolithic column was applied to the on-line extraction of tetracycline residues from milk. An enrichment factor of 15.8 and a good sample clean-up effect were obtained under the optimized conditions. The recoveries of the three spiked milk samples were between 81.7 and 102.5% with relative standard deviations (n = 3) in the range of 2-5%. The limits of detection (S/N = 3) for target compounds were in the range of 3.80-9.03 µg/kg. The results show that the on-line extraction using the restricted-access material-hybrid monolithic column was powerful for food sample pretreatment with high selectivity and good clean-up effect.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tetraciclina
/
Residuos de Medicamentos
/
Leche
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Extracción en Fase Sólida
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Gluconatos
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Lactonas
/
Antibacterianos
Límite:
Animals
Idioma:
En
Revista:
J Sep Sci
Año:
2019
Tipo del documento:
Article