Screening for Neurotoxicity with Microelectrode Array.
Curr Protoc Toxicol
; 79(1): e67, 2019 02.
Article
en En
| MEDLINE
| ID: mdl-30575314
ABSTRACT
Neurotoxicity and seizurogenic liabilities are difficult to detect using currently available in vitro cytotoxicity assays. This is primarily due to the inherent limitations of these assays to predict adverse neural network disruptions and chemically induced perturbations. Many of these detrimental effects are detected with in vivo studies after substantial time and monetary resources have already been invested. Due to these late-stage unforeseen side effects, the implementation of a reliable high throughput in vitro method for assessing seizure-inducing and neurotoxic compound effects early in the drug discovery process would be ideal. We have developed an in vitro screening tool to identify chemical entities that cause neurotoxic and seizurogenic effects. This article describes the preparation and use of a 48-well microelectrode array (MEA) platform along with custom data analysis algorithms and commercially available analysis tools to screen for neurotoxic liabilities and seizurogenic effects using recorded spike file data generated from cryogenically preserved rat cortical neurons. © 2018 by John Wiley & Sons, Inc.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Convulsiones
/
Síndromes de Neurotoxicidad
/
Ensayos Analíticos de Alto Rendimiento
/
Neuronas
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
Idioma:
En
Revista:
Curr Protoc Toxicol
Año:
2019
Tipo del documento:
Article