The Salmonella Enteritidis TolC outer membrane channel is essential for egg white survival.
Poult Sci
; 98(5): 2281-2289, 2019 May 01.
Article
en En
| MEDLINE
| ID: mdl-30624709
ABSTRACT
Salmonella Enteritidis has developed the potential to contaminate eggs by surviving in the antimicrobial environment of the hen's egg white. This has led to a worldwide pandemic of foodborne salmonellosis infections in humans due to the consumption of contaminated eggs and egg-derived products. The molecular mechanisms of Salmonella Enteritidis egg white survival are not fully clear. Using in vivo expression technology and promoter-reporter fusions we showed that the promoter of the tolC gene, encoding the TolC outer membrane channel that is used by multidrug efflux pumps to export harmful molecules and to secrete bacterial products, is activated by egg white at the chicken body temperature. Using a Salmonella Enteritidis tolC deletion mutant we showed that TolC has an important role in egg white survival. Chromatographic separation techniques and subsequent testing of antimicrobial activities of separated egg white fractions led to the identification of ovotransferrin as the egg white antimicrobial factor which is capable of inhibiting growth of a tolC deletion strain but not the wild type strain. We provide evidence that TolC protects Salmonella Enteritidis against ovotransferrin-mediated growth inhibition in egg white.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de las Aves de Corral
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Salmonella enteritidis
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Salmonelosis Animal
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Proteínas de la Membrana Bacteriana Externa
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Pollos
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Clara de Huevo
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Poult Sci
Año:
2019
Tipo del documento:
Article
País de afiliación:
Bélgica