Differentiation of CD45/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro.
Int J Mol Med
; 43(3): 1128-1138, 2019 Mar.
Article
en En
| MEDLINE
| ID: mdl-30628669
ABSTRACT
Side population (SP) cells are a small subpopulation of cells found in many mammalian tissues and organs, identified by their capacity to efflux Hoechst 33342 dye. They are enriched for stem/progenitor cell activity. SP cells isolated from the adult mouse lung can be separated into a CD45+ subset (bone marrowderived) and a CD45 subset that can be subdivided into CD31 and CD31+ subpopulations. CD45/CD31 lung SP (LSP) cells are known to be mesenchymal stem cells. However, CD45/CD31+ LSP cells are not fully characterized. In the present study, it was found that CD45/CD31+ LSP cells were able to form colonies. Based on the expression of vascular endothelial growth factor receptor 2 (VEGFR2), these cells were separated into VEGFR2 and VEGFR2+ cells. The CD45/CD31+/VEGFR2 LSP cells expressed genes characteristic of smooth muscle and endothelial progenitors, and were able to differentiate into smooth muscle and endothelial cells in vitro. The CD45/CD31+/VEGFR2+ LSP cells expressed genes characteristic of endothelial progenitors and gave rise to endothelial cells, although not smooth muscle, in vitro. The data demonstrate that CD45/CD31+/VEGFR2 LSP cells differentiated into CD45/CD31+/VEGFR2+ LSP cells and then endothelial cells, indicating that CD45/CD31+/VEGFR2+ LSP cells are likely to be derived from CD45/CD31+/VEGFR2 LSP cells. Taken together, the results suggest that CD45/CD31+ LSP cells can be separated into CD45/CD31+/VEGFR2 LSP cells, which may be progenitors of endothelial and smooth muscle, whereas CD45/CD31+/VEGFR2+ LSP cells may serve as late commitment endothelial progenitors in the adult mouse lung.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
/
Miocitos del Músculo Liso
/
Células Endoteliales
/
Células de Población Lateral
/
Pulmón
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Int J Mol Med
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Año:
2019
Tipo del documento:
Article