Volumetric two-photon microscopy with a non-diffracting Airy beam.
Opt Lett
; 44(2): 391-394, 2019 Jan 15.
Article
en En
| MEDLINE
| ID: mdl-30644908
ABSTRACT
We demonstrate a volumetric two-photon microscopy (TPM) using the non-diffracting Airy beam as illumination. Direct mapping of the imaging trajectory shows that the Airy beam extends the axial imaging range around six times longer than a traditional Gaussian beam does along the propagation direction, while maintaining a comparable lateral width. Benefiting from its non-diffracting nature, the TPM with Airy beam illumination is able not only to capture a volumetric image within a single frame, but also to acquire image structures behind a strongly scattered medium. The volumetric specimen is mapped layer by layer under Gaussian mode, while the three-dimensional structure is projected to a single two-dimensional image under Airy mode, leading to a significantly increased acquisition speed. The performance of the TPM is evaluated employing a phantom of agarose gel imbedding fluorescent beads as well as a mouse brain slice. Finally, we showcase the penetration ability of the developed Airy TPM by imaging through a scattering environment.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fotones
/
Fenómenos Ópticos
/
Microscopía
Límite:
Animals
Idioma:
En
Revista:
Opt Lett
Año:
2019
Tipo del documento:
Article