A ratiometric fluorescent probe for lysosomal hypochlorous acid based on through-bond energy transfer strategy.
Anal Chim Acta
; 1052: 124-130, 2019 Apr 04.
Article
en En
| MEDLINE
| ID: mdl-30685030
ABSTRACT
In this paper, we synthesized a ratiometric fluorescence probe (IRh-Ly) for lysosomal hypochlorous acid (HOCl) by adopting a through-bond energy transfer (TBET) strategy on rhodamine-imidazo[1,5-a]pyridine platform. IRh-Ly showed brilliant selectivity, rapid response for HOCl. The probe also exhibited high sensitivity with the detection limit calculated to be 10.2â¯nM. Moreover, we demonstrated its successful application of detecting lysosomal HOCl in living RAW264.7â¯cells. Notably, the morpholine was integrated into the fluorescent probe IRh-Ly and the results revealed that IRh-Ly could target lysosome and detect the lysosomal HOCl. All the unique features made IRh-Ly particularly suitable for ratiometric HOCl detection and bio-imaging applications.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácido Hipocloroso
/
Transferencia de Energía
/
Colorantes Fluorescentes
/
Lisosomas
Límite:
Animals
Idioma:
En
Revista:
Anal Chim Acta
Año:
2019
Tipo del documento:
Article