Microbial exopolymer-capped selenium nanowires - Towards new antibacterial, antibiofilm and arbovirus vector larvicides?
J Photochem Photobiol B
; 192: 55-67, 2019 Mar.
Article
en En
| MEDLINE
| ID: mdl-30685584
ABSTRACT
Arboviral diseases and microbial pathogens resistant to commercially available drugs are on the rise. Herein, a facile microbial-based approach was developed to synthesize selenium nanowires (Se NWs) using microbial exopolymer (MEP) extracted from the Bacillus licheniformis (probiotic bacteria). MEP-Se NWs were characterized using UV-Visible, XRD, FTIR, HR-TEM, FE-SEM and EDX. An UV-Visible peak was detected at 330â¯nm while XRD spectrum data pointed out the crystalline nature of MEP-Se NWs. FTIR spectrum revealed functional groups with strong absorption peaks in the range 3898.52-477.97â¯cm-1. FE-SEM and HR-TEM revealed that the obtained structures were nanowires of 10-30â¯nm diameter. Se presence was confirmed by EDX analysis. MEP-Se NWs at 100⯵g/ml highly suppressed the growth of both Gram (-) and Gram (+) bacteria. Further, microscopic analysis evidenced that 75⯵g/ml MEP-Se NWs suppressed biofilm formation. Hemolytic assays showed that MEP-Se NWs were moderately cytotoxic. In addition, LC50 values lower than 10⯵g/ml were estimated testing MEP-Se NWs on both Aedes aegypti and Culex quinquefasciatus 3rd instar larvae. Morphological and histological techniques were used to elucidate on the damages triggered in mosquito tissues, with special reference to midgut, post-exposure to MEP-Se NWs. Therefore, based on our findings, MEP-Se NWs can be considered for entomological and biomedical applications, with special reference to the management of biofilm forming microbial pathogens and arbovirus mosquito vectors.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Selenio
/
Nanocables
/
Matriz Extracelular de Sustancias Poliméricas
Límite:
Animals
Idioma:
En
Revista:
J Photochem Photobiol B
Asunto de la revista:
BIOLOGIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
India