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Genotyping and cytomorphological subtyping of lung adenocarcinoma based on liquid-based cytology.
Tanaka, Ryota; Sakamoto, Norihiko; Suzuki, Hitomi; Tachibana, Keisei; Ohtsuka, Kouki; Kishimoto, Koji; Fujiwara, Masachika; Kamma, Hiroshi; Shibahara, Junji; Kondo, Haruhiko.
Afiliación
  • Tanaka R; Department of Surgery, Kyorin University School of Medicine, Tokyo, Japan.
  • Sakamoto N; Department of Pathology, Kyorin University School of Medicine, Tokyo, Japan.
  • Suzuki H; Department of Pathology, Kyorin University School of Medicine, Tokyo, Japan.
  • Tachibana K; Department of Surgery, Kyorin University School of Medicine, Tokyo, Japan.
  • Ohtsuka K; Department of Clinical Laboratory, Kyorin University School of Medicine, Tokyo, Japan.
  • Kishimoto K; Department of Pathology, Kyorin University School of Medicine, Tokyo, Japan.
  • Fujiwara M; Department of Pathology, Kyorin University School of Medicine, Tokyo, Japan.
  • Kamma H; Department of Pathology, Kyorin University School of Medicine, Tokyo, Japan.
  • Shibahara J; Department of Pathology, Kyorin University School of Medicine, Tokyo, Japan.
  • Kondo H; Department of Surgery, Kyorin University School of Medicine, Tokyo, Japan.
Diagn Cytopathol ; 47(6): 564-570, 2019 Jun.
Article en En | MEDLINE | ID: mdl-30741488
ABSTRACT

BACKGROUND:

Liquid-based cytology (LBC) samples allow immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and molecular testing of nucleic acids to be performed in the remaining fixed cells. The current study aimed to examine the relationship between gene mutational status and cytomorphological features in primary lung adenocarcinoma (ADC) using LBC materials.

METHODS:

Forty consecutive patients with primary lung ADC underwent surgical resection in our hospital. Cytological material was obtained by scraping the cut-surface of the lesion, and samples were fixed and stored as LBC materials using CytoRich Red. Epidermal growth factor receptor (EGFR) and Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations, anaplastic lymphoma kinase (ALK), and c-ros oncogene 1 (ROS1) gene rearrangements were detected, and cytomorphological studies were performed.

RESULTS:

Twenty cases (50%) were positive for EGFR mutation and four (10%) were positive for KRAS mutation. ALK gene rearrangement was identified in one case (2.5%) by IHC and FISH, and ROS1 gene rearrangement was identified in one case (2.5%) by IHC and real-time polymerase chain reaction. The KRAS-positive group included higher proportions of cases with an inflammatory background (100%), predominantly papillary architecture (75%), and papillary-type ADC pattern (75%) compared with the EGFR-positive group and the other group, which included ALK and ROS1 gene rearrangements.

CONCLUSIONS:

LBC material is suitable for use in molecular testing. Differences in major gene aberrations detected by this method might predict specific cytomorphological features.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Genotipaje / Adenocarcinoma del Pulmón / Pulmón Tipo de estudio: Prognostic_studies Límite: Aged / Female / Humans / Male Idioma: En Revista: Diagn Cytopathol Asunto de la revista: PATOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Genotipaje / Adenocarcinoma del Pulmón / Pulmón Tipo de estudio: Prognostic_studies Límite: Aged / Female / Humans / Male Idioma: En Revista: Diagn Cytopathol Asunto de la revista: PATOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA