Two-photon PSF-engineered image scanning microscopy.
Opt Lett
; 44(4): 895-898, 2019 Feb 15.
Article
en En
| MEDLINE
| ID: mdl-30768013
ABSTRACT
We present two-photon fluorescence image scanning microscopy (ISM) with engineered excitation and detection point-spread-functions enabling 3D imaging in a single 2D scan. This demonstration combines excitation using a holographic multispot array of focused femtosecond pulses with a high-efficiency single-helix PSF phase mask detection. Camera detection along with a multiview reconstruction algorithm allows volumetric imaging of biological samples over a depth of field spanning more than 1500 nm with an axial resolution of better than 400 nm. The nonlinear two-photon process improves sectioning and the inherent longer wavelengths increase the penetration depth in scattering samples. Our method extends the performance of 3D ISM towards thicker biological samples.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fotones
/
Imagenología Tridimensional
/
Ingeniería
/
Microscopía Fluorescente
Tipo de estudio:
Clinical_trials
Idioma:
En
Revista:
Opt Lett
Año:
2019
Tipo del documento:
Article