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Granulysin- and granzyme-dependent elimination of myeloid cells by therapeutic ova-specific type 1 regulatory T cells.
Guipouy, Delphine; Gertner-Dardenne, Julie; Pfajfer, Laurène; German, Yolla; Belmonte, Nathalie; Dupré, Loïc.
Afiliación
  • Guipouy D; INSERM, UMR1043, Centre de Physiopathologie de Toulouse Purpan, Purpan University Hospital, Toulouse, France.
  • Gertner-Dardenne J; Université Toulouse III Paul-Sabatier, Toulouse, France.
  • Pfajfer L; CNRS, UMR5282, Toulouse, France.
  • German Y; TxCell, Allée de la Nertière, Valbonne Sophia-Antipolis, France.
  • Belmonte N; TxCell, Allée de la Nertière, Valbonne Sophia-Antipolis, France.
  • Dupré L; INSERM, UMR1043, Centre de Physiopathologie de Toulouse Purpan, Purpan University Hospital, Toulouse, France.
Int Immunol ; 31(4): 239-250, 2019 03 28.
Article en En | MEDLINE | ID: mdl-30778577
The intrinsic immunosuppressive properties of regulatory T (Treg) cells can be harnessed for therapeutic approaches aiming at down-modulating harmful immune reactions. In this context, expanded type 1 Treg cells (Tr1 cells) specific for ovalbumin (ova-Tr1 cells) have been tested for clinical efficacy in the treatment of autoimmune disorders such as refractory Crohn's disease (CD). The clinical use of these therapeutic products warrants exploration of their mechanism of action. Here, we identified a relationship between the CD activity index and the expression of lytic molecules by the ova-Tr1 cells administered in the previously reported First-in-Man study [Crohn's And Treg cells Study 1 (CATS1) study]. Accordingly, ova-Tr1 cells were found to carry granules containing high levels of lytic molecules, including multiple granzymes and granulysin. These cells displayed a T-cell receptor (TCR)-independent cytotoxic activity, which was preferentially directed toward myeloid cell lines and monocyte-derived dendritic cells. Upon contact with myeloid cells, ova-Tr1 cells induced their apoptosis via a perforin-independent and a granulysin/granzyme-dependent mechanism. As compared to CD8+ cytotoxic T cells, ova-Tr1 cells required more time to lyse target cells and displayed a more gradual lytic activity over time. Notably, this activity was sustained over days resulting in the control of myeloid cell populations at a relatively low ratio. Our study reveals that ova-Tr1 cells are endowed with a sustained cytotoxic activity that relies on a unique combination of granulysin and granzymes and that preferentially eliminates myeloid target cells in a TCR-independent manner.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antígenos de Diferenciación de Linfocitos T / Linfocitos T Reguladores / Linfocitos T CD8-positivos / Células Mieloides / Granzimas Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antígenos de Diferenciación de Linfocitos T / Linfocitos T Reguladores / Linfocitos T CD8-positivos / Células Mieloides / Granzimas Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido