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Bright sub-20-nm cathodoluminescent nanoprobes for electron microscopy.
Prigozhin, Maxim B; Maurer, Peter C; Courtis, Alexandra M; Liu, Nian; Wisser, Michael D; Siefe, Chris; Tian, Bining; Chan, Emory; Song, Guosheng; Fischer, Stefan; Aloni, Shaul; Ogletree, D Frank; Barnard, Edward S; Joubert, Lydia-Marie; Rao, Jianghong; Alivisatos, A Paul; Macfarlane, Roger M; Cohen, Bruce E; Cui, Yi; Dionne, Jennifer A; Chu, Steven.
Afiliación
  • Prigozhin MB; Department of Physics, Stanford University, Stanford, CA, USA.
  • Maurer PC; Department of Physics, Stanford University, Stanford, CA, USA.
  • Courtis AM; Department of Chemistry, University of California at Berkeley, Berkeley, CA, USA.
  • Liu N; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
  • Wisser MD; School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
  • Siefe C; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
  • Tian B; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
  • Chan E; Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Song G; Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Fischer S; Department of Radiology, Stanford University, Stanford, CA, USA.
  • Aloni S; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, China.
  • Ogletree DF; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
  • Barnard ES; Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Joubert LM; Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Rao J; Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Alivisatos AP; CSIF Beckman Center, Stanford University, Stanford, CA, USA.
  • Macfarlane RM; EM Unit, Central Analytical Facilities, Stellenbosch University, Stellenbosch, South Africa.
  • Cohen BE; Department of Radiology, Stanford University, Stanford, CA, USA.
  • Cui Y; Department of Chemistry, University of California at Berkeley, Berkeley, CA, USA.
  • Dionne JA; Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Chu S; Department of Materials Science and Engineering, University of California, Berkeley, CA, USA.
Nat Nanotechnol ; 14(5): 420-425, 2019 05.
Article en En | MEDLINE | ID: mdl-30833691
ABSTRACT
Electron microscopy has been instrumental in our understanding of complex biological systems. Although electron microscopy reveals cellular morphology with nanoscale resolution, it does not provide information on the location of different types of proteins. An electron-microscopy-based bioimaging technology capable of localizing individual proteins and resolving protein-protein interactions with respect to cellular ultrastructure would provide important insights into the molecular biology of a cell. Here, we synthesize small lanthanide-doped nanoparticles and measure the absolute photon emission rate of individual nanoparticles resulting from a given electron excitation flux (cathodoluminescence). Our results suggest that the optimization of nanoparticle composition, synthesis protocols and electron imaging conditions can lead to sub-20-nm nanolabels that would enable high signal-to-noise localization of individual biomolecules within a cellular context. In ensemble measurements, these labels exhibit narrow spectra of nine distinct colours, so the imaging of biomolecules in a multicolour electron microscopy modality may be possible.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microscopía Electrónica de Transmisión / Nanopartículas / Colorantes Fluorescentes Idioma: En Revista: Nat Nanotechnol Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microscopía Electrónica de Transmisión / Nanopartículas / Colorantes Fluorescentes Idioma: En Revista: Nat Nanotechnol Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos