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Comparison of Two PCR Assays for Trichomonas vaginalis.
Noh, Chang-Suk; Kim, Sang-Su; Park, Sung-Yul; Moon, Hong-Sang; Hong, Yeonchul; Ryu, Jae-Sook.
Afiliación
  • Noh CS; Department of Internal Medicine, Hallym University Hangang Sacred Heart Hospital, Seoul 07247, Korea.
  • Kim SS; Department of Environmental Biology and Medical Parasitology, Hanyang University College of Medicine, Seoul 04763, Korea.
  • Park SY; Department of Biomedical Science, Hanyang University Graduate School of Biomedical Science and Engineering, Hanyang University College of Medicine, Seoul 04763, Korea.
  • Moon HS; Department of Urology, Hanyang University College of Medicine, Seoul 04763, Korea.
  • Hong Y; Department of Urology, Hanyang University College of Medicine, Seoul 04763, Korea.
  • Ryu JS; Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu 41944, Korea.
Korean J Parasitol ; 57(1): 27-31, 2019 Feb.
Article en En | MEDLINE | ID: mdl-30840796
ABSTRACT
PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de la Próstata / Tricomoniasis / Trichomonas vaginalis / Reacción en Cadena de la Polimerasa / Técnicas de Diagnóstico Molecular Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Humans / Male Idioma: En Revista: Korean J Parasitol Asunto de la revista: PARASITOLOGIA Año: 2019 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de la Próstata / Tricomoniasis / Trichomonas vaginalis / Reacción en Cadena de la Polimerasa / Técnicas de Diagnóstico Molecular Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Humans / Male Idioma: En Revista: Korean J Parasitol Asunto de la revista: PARASITOLOGIA Año: 2019 Tipo del documento: Article