Quantitative Analysis of Intracellular Ca<sup>2+</sup> Release and Contraction in hiPSC-Derived Vascular Smooth Muscle Cells.

Halaidych, Oleh V; Cochrane, Amy; van den Hil, Francijna E; Mummery, Christine L; Orlova, Valeria V

Quantitative Analysis of Intracellular Ca²⁺ Release and Contraction in hiPSC-Derived Vascular Smooth Muscle Cells.

Halaidych, Oleh V; Cochrane, Amy; van den Hil, Francijna E; Mummery, Christine L; Orlova, Valeria V.

Afiliación

Halaidych OV; Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands.
Cochrane A; Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands.
van den Hil FE; Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands.
Mummery CL; Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands.
Orlova VV; Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands. Electronic address: v.orlova@lumc.nl.

Stem Cell Reports ; 12(4): 647-656, 2019 04 09.

Article en En | MEDLINE | ID: mdl-30853373

Asunto(s)

Calcio/metabolismo; Células Madre Pluripotentes Inducidas/citología; Células Madre Pluripotentes Inducidas/metabolismo; Músculo Liso Vascular/citología; Músculo Liso Vascular/metabolismo; Miocitos del Músculo Liso/metabolismo; Diferenciación Celular; Células Cultivadas; Humanos; Espacio Intracelular/metabolismo; Contracción Muscular; Cresta Neural/citología; Cresta Neural/metabolismo

Palabras clave

CellProfiler; LC_Pro plugin for ImageJ; automated image analysis; cell tracking; contraction; human induced pluripotent stem cells (hiPSCs); microfluidics; neural crest-derived vascular smooth muscle cells (NC-SMCs); real-time intracellular Ca(2+) release in vSMCs; vascular smooth muscle cells (vSMCs)

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Miocitos del Músculo Liso / Células Madre Pluripotentes Inducidas / Músculo Liso Vascular Límite: Humans Idioma: En Revista: Stem Cell Reports Año: 2019 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos

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