A rapid, sensitive and inexpensive method for detection of grapevine red blotch virus without tissue extraction using loop-mediated isothermal amplification.
Arch Virol
; 164(5): 1453-1457, 2019 May.
Article
en En
| MEDLINE
| ID: mdl-30895404
ABSTRACT
Grapevine red blotch virus (GRBV) is an emerging virus of significant viticultural importance throughout North America. Here, we report the development of a simple protocol for point-of-use detection of GRBV. Extraction of nucleic acids is not required; instead, the whole intact plant can simply be pricked with a sterile pipette tip, which is then incubated in sterile distilled water to provide the sample template in a loop-mediated isothermal amplification (LAMP) reaction. This method is 10,000 times more sensitive than conventional PCR, costs under a dollar per sample, and can be completed from sampling to readout in just over half an hour.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de las Plantas
/
ADN Viral
/
Geminiviridae
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Técnicas de Amplificación de Ácido Nucleico
/
Vitis
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Arch Virol
Año:
2019
Tipo del documento:
Article
País de afiliación:
México