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Mucin adsorption on vaterite CaCO3 microcrystals for the prediction of mucoadhesive properties.
Balabushevich, Nadezhda G; Kovalenko, Ekaterina A; Mikhalchik, Elena V; Filatova, Lyubov Y; Volodkin, Dmitry; Vikulina, Anna S.
Afiliación
  • Balabushevich NG; Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1-3, 119991 Moscow, Russia.
  • Kovalenko EA; Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1-3, 119991 Moscow, Russia. Electronic address: sholina-katya@mail.ru.
  • Mikhalchik EV; Federal Research and Clinical Centre of Physical-Chemical Medicine, Malaya Pirogovskaya 1A, 119992 Moscow, Russia. Electronic address: lemik2007@yandex.ru.
  • Filatova LY; Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1-3, 119991 Moscow, Russia.
  • Volodkin D; Lomonosov Moscow State University, Department of Chemistry, Leninskiye Gory 1-3, 119991 Moscow, Russia; Nottingham Trent University, School of Science and Technology, Clifton Lane, NG11 8NS Nottingham, UK. Electronic address: dmitry.volodkin@ntu.ac.uk.
  • Vikulina AS; Nottingham Trent University, School of Science and Technology, Clifton Lane, NG11 8NS Nottingham, UK; Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Am Mühlenberg 13, 14476 Potsdam-Golm, Germany. Electronic address: anna.vikulina@izi-bb.fraunhofer.de.
J Colloid Interface Sci ; 545: 330-339, 2019 Jun 01.
Article en En | MEDLINE | ID: mdl-30901672
ABSTRACT
Porous vaterite CaCO3 crystals are widely used as containers for drug loading and as sacrificial templates to assemble polymer-based nano- and micro-particles at mild conditions. Special attention is paid nowadays to mucosal delivery where the glycoprotein mucin plays a crucial role as a main component of a mucous. In this work mucoadhesive properties of vaterite crystals have been tested by investigation of mucin binding to the crystals as a function of (i) time, (ii) glycoprotein concentration, (iii) adsorption conditions and (iv) degree of mucin desialization. Mucin adsorption follows Bangham equation indicating that diffusion into crystal pores is the rate-limiting step. Mucin strongly binds to the crystals (ΔG = -35 ±â€¯4 kJ mol-1) via electrostatic and hydrophobic interactions forming a gel and thus giving the tremendous mucin mass content in the crystals of up to 16%. Despite strong intermolecular mucin-mucin interactions, pure mucin spheres formed after crystal dissolution are unstable. However, introduction of protamine, actively used for mucosal delivery, makes the spheres stable via additional electrostatic bonding. The results of this work indicate that the vaterite crystals are extremely promising carriers for mucosal drug delivery and for development of test-systems for the analysis of the mucoadhesion.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: J Colloid Interface Sci Año: 2019 Tipo del documento: Article País de afiliación: Rusia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: J Colloid Interface Sci Año: 2019 Tipo del documento: Article País de afiliación: Rusia