Pregnancy protects against the pro-inflammatory respiratory responses induced by particulate matter exposure.
Chemosphere
; 225: 796-802, 2019 Jun.
Article
en En
| MEDLINE
| ID: mdl-30904759
ABSTRACT
BACKGROUND:
Little is known about the effect of pregnancy on the response to particulate matter. The aim of this study was to determine if pregnancy increases the susceptibility to PM from different sources using a mouse model.METHODS:
Pregnant, eight-week-old C57BL/6J mice were exposed intranasally to 50⯵g of diesel exhaust particles (DEP), iron oxide (Fe2O3) or silica (SiO2) in 50⯵L of saline, or saline alone, on gestational day (E)7.5, E12.5 and E17.5. Groups of non-pregnant mice were exposed on day (D)0, D5 and D10. Biological samples were collected 24â¯h after the last exposure. Serum IL-4 and IL-6 levels were quantified by ELISA. Bronchoalveolar lavage (BAL) fluid was collected for inflammatory cells counts and assessment of IFN-É£, IL-4, IL-5, IL-6, IL-8 and IL-10 levels by ELISA. The spleen and thymus were also collected and the percentage of B cells and CD4+, CD8+ and CD4+CD25â¯+â¯T cells were determined by flow cytometry.RESULTS:
Exposure to silica caused an influx of lymphocytes, eosinophils and neutrophils into the lung. The magnitude of this response was suppressed by pregnancy. Pregnancy also enhanced the production of CD4+CD25â¯+â¯T cells in response to DEP and silica exposure.CONCLUSIONS:
Collectively, our data suggest that pregnancy reduces the inflammatory response to silica and alters the immune response to DEP. These responses were accompanied by pregnancy related changes including increased IL-4 production, reduced IL-8 production and an increase in the proportion of CD4+CD25â¯+â¯T cells in response to PM exposure.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sistema Respiratorio
/
Emisiones de Vehículos
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Embarazo
/
Material Particulado
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Inflamación
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Chemosphere
Año:
2019
Tipo del documento:
Article
País de afiliación:
Australia
Pais de publicación:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM