Hypoxia protects against the cell death triggered by oxovanadium-galactomannan complexes in HepG2 cells.
Cell Mol Biol Lett
; 24: 18, 2019.
Article
en En
| MEDLINE
| ID: mdl-30949212
ABSTRACT
BACKGROUND:
Polysaccharides from various sources have been used in traditional medicine for centuries. The beneficial pharmacological effects of plant-derived polysaccharides include anti-tumor activity.METHODS:
Here, we evaluated the anti-cancer effect of the MSAGMVO complex under hypoxic conditions (1% oxygen). MSAGMVO is a complex of the hydrolysate of galactomannan (MSAGM) from Schizolobium amazonicum with oxovanadium (IV/V). The hepatocellular carcinoma (HCC) cell line HepG2 was selected as HCC are one of the most hypoxic solid tumors.RESULTS:
Our results showed that the strong apoptotic activity of MSAGMVO observed in HepG2 cells under normoxic conditions was completely lost under hypoxic conditions. We found a dynamic balance between the pro- and anti-apoptotic members of the Bcl-2 protein family. The expressions of anti-apoptotic Mcl-1 and Bcl-XL increased in hypoxia, whereas the expression of pro-apoptotic Bax decreased. MSAGMVO strongly induced autophagy, which was previously characterized as a pro-survival mechanism in hypoxia. These results demonstrate total elimination of the anti-cancer activity of MSAGMVO with activation of autophagy under conditions of hypoxia.CONCLUSION:
Although this study is a proof-of-concept of the impact of hypoxia on the potential of polysaccharides, further study is encouraged. The anti-tumor activity of polysaccharides could be achieved in normoxia or through raising the activity of the immune system. In addition, combination strategies for therapy with anti-autophagic drugs could be proposed.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vanadatos
/
Citoprotección
/
Mananos
Límite:
Humans
Idioma:
En
Revista:
Cell Mol Biol Lett
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article
País de afiliación:
Brasil