Isolation, structure and activity of a novel water-soluble polysaccharide from Dioscorea opposita Thunb.
Int J Biol Macromol
; 133: 1201-1209, 2019 Jul 15.
Article
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| MEDLINE
| ID: mdl-30986466
ABSTRACT
A previously unknown, water-soluble polysaccharide, named DOTP-B, was isolated from the roots of the plant Dioscorea opposita Thunb, a well-known edible and medicinal plant in China. DOTP-B was found to be neutral in charge, with an average molecular weight of 5623â¯Da. Monosaccharide composition analysis by GC-MS revealed that DOTP-B was a hetero-polysaccharide consisting of glucose and galactose at a molar ratio of 14.61.0. Structural features of DOTP-B were investigated with a combination of chemical and instrumental methods, including complete acid hydrolysis, periodate oxidation, methylation, GC-MS, FTIR and several NMR spectra. Highly correlated results demonstrated that the main chain of DOTP-B consisted of â4)-α-d-glc(1â¯ââ¯residues, with about 6% internal â6)-ß-d-gal(1â¯ââ¯residues. The antioxidant activity of DOTP-B was also evaluated as the EC50 values against DPPH and PTIO radicals were 2.1⯱â¯0.1â¯mg/mL and 1.6⯱â¯0.1â¯mg/mL, respectively. Therefore, the polysaccharide DOTP-B could be possibly developed as a promising natural antioxidant for application in medical and food industries.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos
/
Agua
/
Dioscorea
/
Antioxidantes
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2019
Tipo del documento:
Article
País de afiliación:
China