Protective effects of tyndallized Lactobacillus acidophilus IDCC 3302 against UVBinduced photodamage to epidermal keratinocytes cells.
Int J Mol Med
; 43(6): 2499-2506, 2019 Jun.
Article
en En
| MEDLINE
| ID: mdl-31017257
ABSTRACT
Photoaging is a consequence of chronic exposure to ultraviolet (UV) radiation and results in skin damage. In this study, whether tyndallizate of the probiotic bacterium Lactobacillus acidophilus IDCC 3302 (ACT 3302) can protect against UVBinduced photodamage to the skin was investigated. For this, HaCaT keratinocytes were used as a model for skin photoaging. HaCaT cells were treated with ACT 3302 prior to UVB exposure and skin hydration factors and matrix metalloproteinase (MMP)1, MMP2, and MMP9 levels in the culture supernatant were evaluated by ELISA. The protective effects of ACT 3302 against UVBinduced oxidative stress in HaCaT cells was also assessed by measuring superoxide dismutase and catalase activity and detecting the expression of proinflammatory cytokineencoding genes and mitogenactivated protein kinase (MAPK) signaling components by reverse transcriptionquantitative polymerase chain reaction and western blotting, respectively. UVB exposure increased MMP expression and MAPK activation; these changes were attenuated by pretreatment with ACT 3302. Treatment with ACT 3302 prior to UVB exposure also attenuated inflammation. These results demonstrate that tyndallized ACT 3302 can mitigate photodamage to the skin induced by UVB radiation through the suppression of MMPs and could therefore be used clinically to prevent wrinkle formation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rayos Ultravioleta
/
Queratinocitos
/
Envejecimiento de la Piel
/
Probióticos
/
Lactobacillus acidophilus
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Int J Mol Med
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Año:
2019
Tipo del documento:
Article