On-off-on relay fluorescence recognition of ferric and fluoride ions based on indicator displacement in living cells.
Anal Chim Acta
; 1066: 112-120, 2019 Aug 20.
Article
en En
| MEDLINE
| ID: mdl-31027525
ABSTRACT
A new boronic acid derivative functionalized with a 4-(3-(4-(4,5-diphenyl-1H-imidazole-2-yl)phenyl)-1,2,4-oxadiazol-5-yl)phenyl (IOP) moiety was synthesized for use as a sequential "on-off-on"-type relay fluorescence probe for Fe3+ ions and F- ions with high selectivity and sensitivity under physiological conditions. The introduction of Fe3+ to IOP boronic acid (IOPBA) formed an Fe3+IOPBA complex, which led to quenching of the blue fluorescence intensity at 458â¯nm. The lowest-energy conformation of IOPBA was theoretically predicted to adopt an extended structure, and the Fe3+ ion in the Fe3+IOPBA complex was coordinated to two phenyl groups to form a π-complex. Upon addition of F- to the Fe3+IOPBA complex, the original fluorescence was recovered due to formation of [FeF6]3â, resulting in "on-off-on"-type sensor behavior. IOPBA showed high selectivity towards Fe3+ among other cations. Moreover, the Fe3+IOPBA complex showed specific selectivity towards F-, with other cations and anions not interfering with detection. Both sensing processes showed 11 stoichiometry with binding constants of 6.87â¯×â¯106 and 4.49â¯×â¯106â¯mol-1â¯L for Fe3+ with IOPBA and F- with Fe3+IOPBA, respectively. The limits of detection for Fe3+ and F- were 10 and 1â¯nM, respectively. The proposed method was successfully applied in real water samples. Furthermore, the probe had low cytotoxicity and was successfully used as a bioimaging reagent to detect intracellular Fe3+ and F- in living HeLa cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos Férricos
/
Fluorescencia
/
Fluoruros
Límite:
Humans
Idioma:
En
Revista:
Anal Chim Acta
Año:
2019
Tipo del documento:
Article
Pais de publicación:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS