Mechanisms of σ54-Dependent Transcription Initiation and Regulation.
J Mol Biol
; 431(20): 3960-3974, 2019 09 20.
Article
en En
| MEDLINE
| ID: mdl-31029702
ABSTRACT
Cellular RNA polymerase is a multi-subunit macromolecular assembly responsible for gene transcription, a highly regulated process conserved from bacteria to humans. In bacteria, sigma factors are employed to mediate gene-specific expression in response to a variety of environmental conditions. The major variant σ factor, σ54, has a specific role in stress responses. Unlike σ70-dependent transcription, which often can spontaneously proceed to initiation, σ54-dependent transcription requires an additional ATPase protein for activation. As a result, structures of a number of distinct functional states during the dynamic process of transcription initiation have been captured using the σ54 system with both x-ray crystallography and cryo electron microscopy, furthering our understanding of σ54-dependent transcription initiation and DNA opening. Comparisons with σ70 and eukaryotic polymerases reveal unique and common features during transcription initiation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacterias
/
ARN Polimerasa Sigma 54
/
Iniciación de la Transcripción Genética
Idioma:
En
Revista:
J Mol Biol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Reino Unido