PCR-free and chemistry-based technology for miR-21 rapid detection directly from tumour cells.
Talanta
; 200: 51-56, 2019 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-31036216
ABSTRACT
miRNAs are well known for being implicated in a myriad of biological situations, including those related to serious diseases. Amongst miRNAs, miRNA-21 has the spotlight as it is reported to be up-regulated in multiple severe pathological conditions, being its quantification a key point in medicine. To date, most of the techniques for miRNA quantification have shown to be less effective than expected; thus, we herein present a novel, rapid, cost-effective, robust and PCR-free approach, based on dynamic chemistry, for the identification and quantification of miRNA directly from tumour cells using both FACS and a fluorescent microplate. This dynamic chemistry novel application involves bead based reagents and allows quantifying the number of miR-21 molecules presented in MDA-MB-468 and H1975 tumour cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
MicroARNs
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Talanta
Año:
2019
Tipo del documento:
Article
País de afiliación:
España