Fluidic resistance control enables high-throughput establishment of mixed-species biofilms.
Biotechniques
; 66(5): 235-239, 2019 05.
Article
en En
| MEDLINE
| ID: mdl-31050304
ABSTRACT
Bacteria often live in communities of mixed species embedded in a self-produced extracellular matrix of polysaccharides, proteins and DNA, termed biofilms. The BioFlux microfluidic flow system is useful for studying biofilm formation in different media under flow. However, analyzing the architecture and maturation of biofilms under flow requires a proper seeding, which can prove difficult when working with bacteria of different sizes, motile bacteria or aiming for a high number of replicates. Here we developed an efficient protocol that exploits viscosity tuning and seeding indicator dyes to improve seeding and allow for high-throughput examination and visualization of consistent mono- and mixed-species biofilm developments under flow.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pseudomonas aeruginosa
/
Biopelículas
/
Microfluídica
/
Microbiota
Idioma:
En
Revista:
Biotechniques
Año:
2019
Tipo del documento:
Article
País de afiliación:
Dinamarca