Your browser doesn't support javascript.
loading
Combined microRNAome and transcriptome analysis of follicular phase and luteal phase in porcine ovaries.
Zhang, Xiaodong; Zhang, Liang; Shang, Jinnan; Tao, Qiangqiang; Tian, Mi; Ma, Yingchun; Xu, Yiliang; Ding, Yueyun; Zhou, Rong; Li, Kui; Yin, Zongjun.
Afiliación
  • Zhang X; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Zhang L; Key Laboratory of Local Animal Genetic Resources Conservation and Bio-Breeding of Anhui Province, Hefei, China.
  • Shang J; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Tao Q; Key Laboratory of Local Animal Genetic Resources Conservation and Bio-Breeding of Anhui Province, Hefei, China.
  • Tian M; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Ma Y; Key Laboratory of Local Animal Genetic Resources Conservation and Bio-Breeding of Anhui Province, Hefei, China.
  • Xu Y; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Ding Y; Key Laboratory of Local Animal Genetic Resources Conservation and Bio-Breeding of Anhui Province, Hefei, China.
  • Zhou R; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Li K; Key Laboratory of Local Animal Genetic Resources Conservation and Bio-Breeding of Anhui Province, Hefei, China.
  • Yin Z; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Reprod Domest Anim ; 54(7): 1018-1025, 2019 Jul.
Article en En | MEDLINE | ID: mdl-31077469
The aim of this study was to explore the expression difference of miRNAs and mRNAs between the follicular phase (FP) and luteal phase (LP) in porcine ovaries and provide a theoretical basis for the research on mammalian reproductive regulation. RNA-Seq and miRNA-Seq were used to identify differentially expressed genes (DEGs) and miRNAs (DEMs) between the FP and LP in ovaries of six sows (3-year-old Yorkshire pigs with similar weights and same parities). Bioinformatic analysis was used to screen potential genes and miRNAs related to porcine ovarian function. Real-time qualitative PCR was used to validate the sequencing results. RNA-Seq results showed that 3,078 genes were up-regulated, and 1,444 genes were down-regulated in the LP compared with the FP, and DEGs were significantly enriched in 242 Gene Ontology (GO) terms and 33 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. miRNA-Seq identified 112 DEMs, of which 25 were up-regulated and 87 were down-regulated in the LP compared with the FP. We obtained 186 intersection genes (IGs) between the 4,522 DEGs and 2,444 target genes predicted from the 112 DEMs. After constructing a miRNA-gene-pathway network, we identified key miRNAs and genes including miR-17-3p, miR-214, miR-221-5p, miR-125b, FGF1, YWHAG, YWHAZ, FDFT1 and DHCR24, which are enriched in Hippo and PI3K-Akt signalling pathways, and various metabolic pathways. These results indicate that these key genes and miRNAs may play important roles in the developmental transition from FP to LP in porcine ovaries and represent candidate targets for further study.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sus scrofa / MicroARNs / Fase Folicular / Fase Luteínica Tipo de estudio: Qualitative_research Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sus scrofa / MicroARNs / Fase Folicular / Fase Luteínica Tipo de estudio: Qualitative_research Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania