[Aconitine ameliorates cardiomyocyte hypertrophy induced by angiotensin â
¡].
Zhongguo Zhong Yao Za Zhi
; 44(8): 1642-1647, 2019 Apr.
Article
en Zh
| MEDLINE
| ID: mdl-31090329
ABSTRACT
This paper was aimed to investigate the inhibitory effect of aconitine(AC) on angiotensin â
¡(Ang â
¡)-induced H9 c2 cell hypertrophy and explore its mechanism of action. The model of hypertrophy was induced by Ang â
¡(1×10-6 mol·L-1),and cardiomyocytes were incubated with different concentrations of AC. Western blot was used to quantify the protein expression levels of atrial natriuretic peptide(ANP),brain natriuretic peptide(BNP),ß-myosin heavy chain(ß-MHC),and α-smooth muscle actin(α-SMA). Real-time quantitative PCR(qRT-PCR) was used to quantify the mRNA expression levels of cardiac hypertrophic markers ANP,BNP and ß-MHC. In addition,the fluorescence intensity of the F-actin marker,an important component of myofibrils,was detected by using laser confocal microscope. AC could significantly reverse the increase of total protein content in H9 c2 cells induced by Ang â
¡; qRT-PCR results showed that AC could significantly inhibit the ANP,BNP and ß-MHC mRNA up-regulation induced by Angâ
¡. Western blot results showed that AC could significantly inhibit the ANP,BNP and ß-MHC protein up-regulation induced by Angâ
¡. In addition,F-actin expression induced by Ang â
¡ could be inhibited by AC,and multiple indicators of cardiomyocyte hypertrophy induced by Ang â
¡ could be down-regulated,indicating that AC may inhibit cardiac hypertrophy by inhibiting the expression of hypertrophic factors,providing new clues for exploring the cardiovascular protection of AC.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aconitina
/
Angiotensina II
/
Miocitos Cardíacos
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2019
Tipo del documento:
Article
País de afiliación:
China