Lacto-N-tetraose synthesis by wild-type and glycosynthase variants of the ß-N-hexosaminidase from Bifidobacterium bifidum.
Org Biomol Chem
; 17(23): 5661-5665, 2019 06 12.
Article
en En
| MEDLINE
| ID: mdl-31094393
ABSTRACT
Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for ß-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum ß-N-hexosaminidase. Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligosacáridos
/
Bifidobacterium bifidum
/
Hexosaminidasas
Idioma:
En
Revista:
Org Biomol Chem
Asunto de la revista:
BIOQUIMICA
/
QUIMICA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Austria
Pais de publicación:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM