Fecal short-chain fatty acid concentrations and dysbiosis in dogs with chronic enteropathy.
J Vet Intern Med
; 33(4): 1608-1618, 2019 Jul.
Article
en En
| MEDLINE
| ID: mdl-31099928
ABSTRACT
BACKGROUND:
Accumulating evidence shows an important relationship between the gastrointestinal (GI) microbiota and host health. Microbial metabolites are believed to play a critical role in host-microbial interactions. Short-chain fatty acids (SCFAs) are major end products of bacterial carbohydrate fermentation in the intestinal tract. Decreased concentrations of SCFAs have been observed in humans with GI disease. However, large-scale clinical data in dogs are lacking. HYPOTHESIS/OBJECTIVE:
To evaluate fecal concentrations of SCFAs and the fecal microbiota in healthy control (HC) dogs and dogs with chronic enteropathy (CE). ANIMALS Forty-nine privately owned HC dogs and 73 dogs with CE.METHODS:
Prospective cohort study. Fecal concentrations of SCFAs were measured using gas chromatography/mass spectrometry. Illumina sequencing and quantitative real-time polymerase chain reaction were utilized to evaluate the fecal microbiota.RESULTS:
Fecal concentrations (median [range] µmol/g of dry matter) of acetate were lower (P = .03) in dogs with CE (185.8 [20.1-1042.1]) than in HC dogs (224.0 [87.7-672.8]). Propionate were also lower (P < .001) in dogs with CE (46.4 [0.4-227.9]) than in HC dogs (105.9 [1.6-266.9]). Moreover, total SCFAs were lower (P = .005) in dogs with CE (268.1 [21.8-1378.2]) than in HC dogs (377.2 [126.6-927.0]). Dysbiosis in dogs with CE was characterized by decreased bacterial diversity and richness, distinct microbial community clustering compared with that in HC dogs, and a higher dysbiosis index. CONCLUSIONS AND CLINICAL IMPORTANCE Dogs with CE had an altered fecal SCFA concentration accompanied by significant changes of the fecal microbiota.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de los Perros
/
Ácidos Grasos Volátiles
/
Disbiosis
/
Microbioma Gastrointestinal
/
Enfermedades Intestinales
Tipo de estudio:
Observational_studies
Límite:
Animals
Idioma:
En
Revista:
J Vet Intern Med
Asunto de la revista:
MEDICINA INTERNA
/
MEDICINA VETERINARIA
Año:
2019
Tipo del documento:
Article