Improved yield and accuracy for DNA extraction in microbiome studies with variation in microbial biomass.
Biotechniques
; 66(6): 285-289, 2019 06.
Article
en En
| MEDLINE
| ID: mdl-31124702
ABSTRACT
A major challenge for microbiome studies is maintaining an even and accurate DNA extraction in the presence of samples with a wide range of bacterial content. Here we compare five DNA extraction methods using replicate stool samples that were diluted to create high and low biomass samples. Our results indicate greater variation in microbiome composition between high and low biomass samples than variation between methods. Many of the extraction methods had reduced yield from low biomass samples; however, our adapted plate column-based extraction method was evenly efficient and captured the largest number of high-quality reads. Based on these results, we have identified a DNA extraction method that ensures adequate yield in metagenomic microbiome studies that have samples with a broad range of bacterial content.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacterias
/
ADN Bacteriano
/
Microbiota
Límite:
Humans
Idioma:
En
Revista:
Biotechniques
Año:
2019
Tipo del documento:
Article
País de afiliación:
Estados Unidos