Synthesis and screening of bead-displayed combinatorial libraries.
Methods Enzymol
; 622: 91-127, 2019.
Article
en En
| MEDLINE
| ID: mdl-31155067
ABSTRACT
The development of faster and less expensive methods to discover bioactive small molecules remains a high priority in chemical biology. This article discusses one alternative to traditional high-throughput screening the synthesis and screening of one bead one compound (OBOC) libraries. Protocols are provided to create and screen libraries of peptoid displayed on TentaGel beads, which is a cheap and relatively straightforward process for the identification of selective protein ligands. However, peptoids bind to proteins with modest affinity in most cases. Therefore, we also describe protocols to create libraries of stiffer oligomers called PICCOs (peptoid-inspired, conformationally constrained oligomers) that have proven to be a superior source of high affinity ligands.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Químicas Combinatorias
/
Peptoides
/
Bibliotecas de Moléculas Pequeñas
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Enzymol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Estados Unidos