LINE-1 methylation in cleft lip tissues: Influence of infant MTHFR c.677C>T genotype.
Oral Dis
; 25(6): 1668-1671, 2019 Sep.
Article
en En
| MEDLINE
| ID: mdl-31161688
ABSTRACT
OBJECTIVE:
To investigate the influence of MTHFR c.677C>T genotype on LINE-1 methylation in lateral and medial tissues from cleft lip (CL).METHODS:
Forty-five consecutive non-syndromic cleft lip with or without cleft palate (nsCL/P) cases were included in the study. Genomic DNA was extracted from tissues at both sides of cleft lip, and LINE-1 methylation was detected by bisulfite conversion and pyrosequencing. MTHFR c.677C>T genotyping was carried out using the TaqMan genotyping assay.RESULTS:
LINE-1 methylation level was significantly higher on medial side of cleft lip compared with lateral side (p = 0.001). This difference was not significantly influenced by the case's sex or cleft type. However, MTHFR c.677C>T genotyping revealed that the difference in LINE-1 methylation across cleft lip was restricted to carriers of C allele of MTHFR c.677C>T and was not apparent in TT homozygous cases (p = 0.027).CONCLUSION:
This integrated analysis supports the previous finding of differences in DNA methylation across the two sides of cleft lip and further suggests a possible role of MTHFR c.677C>T genotype in establishing this difference.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Labio Leporino
/
Fisura del Paladar
/
Metilenotetrahidrofolato Reductasa (NADPH2)
Tipo de estudio:
Observational_studies
Límite:
Humans
/
Infant
Idioma:
En
Revista:
Oral Dis
Asunto de la revista:
ODONTOLOGIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Italia